Zyagen sheep UniversalReferenceRNAiscomposedofhighlypureintacttotalRNAisolatedfrommajormaleandfemale sheep tissuesincludingbrain,heart,kidney,liver,lung,skeletalmuscles,pancreas,spleen,stomach,intestine,colon,testis,ovaryanduterus.TotalRNAisextractedusingmodifiedguanidinethiocyanatetechniquewhichallowstherapidisolationoftotalRNAincludingmicroRNAs.Tissuesarecutinverysmallpieces,snapfrozen,grindingtopowder,andhomogenizedinRNAdenaturedsolutionforRNApurification.TheextractedRNAistreatedwithDNase,packedataconcentrationof1mg/ml,andstoredat-80oC
Features:
- HighqualityintactRNAwithoutgenomicDNAcontamination
- Consistentcontrolforstandarddatasetcomparisons
- Extractedfromlargevarietyofmaleandfemale sheep majortissuesforbroadgenecoverage
- Madeat industrialscalesto minimizelot-to-lotvariation
QualityControl: TheintegrityofRNAsampleasindicatedbyintactribosomalRNAisverifiedbydenaturedagarosegelelectrophoresis.ThepurityofRNAisassessedbyspectrophotometer(A260/A280:1.9-2.1). ResidualDNAcontaminationistestedbyPCR.
Applications: Reference RNAisidealformicroarrayanalysis,Northernblotting,ribonucleaseprotectionassay,SInucleaseassay,RT-PCR/Q-PCR/RACEanalysis,CDNAsynthesis,RNAdifferentialdisplay,microRNAstudies,andpurificationofmRNAforlibraryconstruction.
Packing/shipping:TotalRNAsampleisroutinelyprovidedinRNase-freewaterataconcentrationof1mg/mlandshippedondryice.
MSDSandCertificateofAnalysisinPDFfiles: ContactZyagenTechnicalSupportat zinfo@zyagen.com