MouseUniversalReferenceiscomposedofhighlypureintacttotalRNAisolatedfromalltissuesofseveraladultmaleandfemaleC57BL/6miceusingmodifiedguanidinethiocyanatetechniqueswhichallowstherapidisolationoftotalRNAincludingmicroRNAs.Tissues(organs)ofanimalswerecutinverysmallpieces,snapfrozen,grindingtopowder,andhomogenizedinRNAdenaturedsolution.TheextractedtissueRNAistreatedwithDNase,packedataconcentrationof1mg/ml,andstoredat-80oC
Features:
- HighqualityintactRNAwithoutgenomicDNAcontamination
- Consistentcontrolforstandarddatasetcomparisons
- Extractedfromallmousetissuesforbroadgenecoverage
- Madeat industrialscalesto minimizelot-to-lotvariation
QualityControl: TheintegrityofRNAsampleasindicatedbyintactribosomalRNAisverifiedbydenaturedagarosegelelectrophoresis.ThepurityofRNAisassessedbyspectrophotometer(A260/A280:1.9-2.1). ResidualDNAcontaminationistestedbyPCR.
Applications: Reference RNAisidealformicroarrayanalysis,Northernblotting,ribonucleaseprotectionassay,SInucleaseassay,RT-PCR/Q-PCR/RACEanalysis,CDNAsynthesis,RNAdifferentialdisplay,microRNAstudies,andpurificationofmRNAforlibraryconstruction.
Packing/shipping:TotalRNAsampleisroutinelyprovidedinRNase-freewaterataconcentrationof1mg/mlandshippedondryice.Samplecanalsobeprovidedin1mMsodiumcitrate,0.1mMEDTA,oranyotherRNAstoragebuffer.
MSDSandCertificateofAnalysisinPDFfiles: ContactZyagenTechnicalSupportat zinfo@zyagen.com