MonkeyBrain,Rhesus MicroRNA(<200nt)isenrichedfromtotalRNAextractedfromfreshlyharvestedtissuesusingclassicalguanidineisothiocyanate–phenol:chloroformextractionmethodandtreatedwithDNasetoremoveresidualDNAcontamination.ThesmallRNAsincluderegulatoryRNAmoleculessuchas microRNA(miRNA)andshortinterferingRNA(siRNA),aswellastRNAand5SrRNA,preciselyquantified,andstoredat-80oC.
QualityControl: TheintegrityofeachRNAsampleisverifiedbydenaturedagarosegelelectrophoresistoconfirmthateachpreparationcontainsintactmicroRNAwithvirtuallynolargeRNAcontamination.ThepurityofRNAisassessedbyspectrophotometer(A260/A280:1.9-2.1).
Applications: MicroRNAisidealformiRNA,siRNA,shRNA,andsnRNAanalysis byrealtimePCR,RT-PCRanalysis,bioanalyzer,NorthernBlotanalysis,RNaseprotectionsassay,primerextension,andexpressionstandardinmicroarrayapplications.
Packing/shipping:TotalRNAsampleisroutinelyprovidedinRNase-freewaterataconcentrationof0.25mg/mlor0.5mg/mlandshippedondryice.Samplecanalsobeprovidedin1mMsodiumcitrate,0.1mMEDTA,oranyotherRNAstoragebuffer.Samplesareshippedondryice.
MSDSandCertificateofAnalysisinPDFfiles: ContactZyagenTechnicalSupportat zinfo@zyagen.com