Background
Wnt-7a belongs to the Wnt family of signaling proteins that play a key role in maintaining the integrity of embryonic and adult tissues. It is expressed in placenta, kidney, testis, uterus, fetal lung and fetal and adult brain. Most Wnt proteins can signal though a mechanism called the canonical Wnt pathway, in which Wnt proteins bind to and activate seven-pass transmembrane receptors of the Frizzled family ultimately leading to the disruption of ¦Â-Catenein degradation. Intracellular accumulation of β-Catenin increases translocation of the protein into the nucleus where it binds to TCF/LEF transcription factors and induces the expression of numerous genes. Increased Wnt/¦Â-Catenein signaling is associated with tumorigenesis in a diverse set of human cancers. However, Wnt-7a/Frizzled-9 signaling has been shown to act as a tumor suppressor in non-small cell lung cancers. Recombinant human Wnt-7a is a 35.5 kDa glycoprotein containing 318 amino acids. Due to glycosylation, Wnt-7a migrates between 40-55 kDa by SDS-PAGE gel under unreduced conditions.
Specifications
Location
LGASIICNKI PGLAPRQRAI CQSRPDAIIV IGEGSQMGLD ECQFQFRNGR WNCSALGERT VFGKELKVGS REAAFTYAII AAGVAHAITA ACTQGNLSDC GCDKEKQGQY HRDEGWKWGG CSADIRYGIG FAKVFVDARE IKQNARTLMN LHNNEAGRKI LEENMKLECK CHGVSGSCTT KTCWTTLPQF RELGYVLKDK YNEAVHVEPV RASRNKRPTF LKIKKPLSYR KPMDTDLVYI EKSPNYCEED PVTGSVGTQG RACNKTAPQA SGCDLMCCGR GYNTHQYARV WQCNCKFHWC CYVKCNTCSE RTEMYTCK
Source
HEK 293 cells
Reactivity
n/a
Preparation
Biological activity was determined by its ability to inhibit Wnt3a induced alkaline phosphatase production in MC3T3-E1 cells. The expected ED50 for this effect is 40-60 ng/mL. Endotoxin level is less than 0.1 ng per μg of WNT-7A (1EU/μg).
Storage
The lyophilized WNT-7A recombinant protein is stable for at least 2 years from date of receipt at -20ËšC. Reconstituted WNT-7A stable for at least 3 months when stored in working aliquots with a carrier protein at -20ËšC. As with any protein, exposing WNT-7A recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Purity
Greater than 80% by SDS-PAGE gel and HPLC analyses.
Disclaimer
This product is for research use only.
Tested Application
n/a
Species Reactivity
n/a
