Background
TWEAK belongs to the TNF family of ligands and signals through TWEAKR also known as TNFRSF12A. TWEAK is expressed in a variety of tissues, including the adult heart, pancreas, skeletal muscle, small intestine, spleen and peripheral blood lymphocytes. TWEAK has the ability to induce NFkB activation and chemokine secretion, and to exert an apoptotic activity in certain cells, such as HT-29 human adenocarcinoma cells when cultured in the presence of IFN-γ. TWEAK also promotes proliferation and migration of endothelial cells. Recombinant human TWEAK is a soluble 17.0 kDa polypeptide (154 amino acid residues) comprising the TNF homologous region of TWEAK and is generated by proteolytic processing of the full length membrane anchored TWEAK protein.
Specifications
Additional Names
TNF-related weak inducer of apoptosis, TNFSF12, DR3LG, Apo3-Ligand
Preparation
Assay #1: The ED50 was determined by the dose - dependent stimulation of IL - 8 production by Human PBMC is less than 10 ng/ml.
Assay #2: TWEAK weakly induces the death of HT29 cells when cultured in the presence of IFN - ?.The ED50 for this effect is between 30 - 45 ng/ml.
Location
MKGRKTRARR AIAAHYEVHP RPGQDGAQAG VDGTVSGWEE ARINSSSPLR YNRQIGEFIV TRAGLYYLYC QVHFDEGKAV YLKLDLLVDG VLALRCLEEF SATAASSLGP QLRLCQVSGL LALRPGSSLR IRTLPWAHLK AAPFLTYFGL FQVH
Source
E. coli
Reactivity
n/a
Tested Application
n/a
Storage
The lyophilized TWEAK recombinant protein is stable for at least 2 years from date of receipt at -20ËšC. Reconstituted TWEAK is stable for at least 3 months when stored in working aliquots with a carrier protein at -20ËšC. As with any protein, exposing TWEAK recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Species Reactivity
n/a
Purity
Greater than 98% by SDS-PAGE gel and HPLC analyses.
Endotoxin level is less than 0.1 ng per μg (1EU/μg).
