Background
TNF-βisapotentmediatorofinflammatoryandimmuneresponses.ItbelongstotheTNFsuperfamilyofligandsandsignalsthroughTNFR1andTNFR2.TNF-βisproducedbyactivatedTandBlymphocytes,andhassimilaractivitiestoTNF-α.LikeTNF-α,TNF-βisinvolvedintheregulationofvariousBIOLOGicalprocessesincudingcellproliferation,differentiation,apoptosis,lipidmetabolism,coagulation,andneurotransmission.TNF-βissecretedasasolublepolypeptide,butcanformheterotrimerswithlymphotoxin-β,whicheffectivelyanchorstheTNF-βtothecellsurface.TNF-βiscytotoxictoawiderangeoftumorcells.RecombinanthumanTNF-βisasecreted172aminoacidprotein(18.6kDa)whichformsanactivenon-disulfidelinkedhomotrimerstructureinsolution.Duetoglycosylation,TNF-βhasanapproximateMWof19.3kDabasedonSDS-PAGEgelamdMassSpectrometry.
Specifications
AdditionalNames
TumorNecrosisFactor-beta,TNFSF1,Lymphotoxin-alpha(LT-alpha)Preparation
TheED50 wasdeterminedbythecytolysisofmurineL929cellsinthepresenceofActinomycinDis?0.05ng/ml,correspondingtoaspecificactivityof?2x107 units/mg.Location
GVGLTPSAAQTARQHPKMHLAHSTLKPAAHLIGDPSKQNSLLWRANTDRAFLQDGFSLSNNSLLVPTSGIYFVYSQVVFSGKAYSPKATSSPLYLAHEVQLFSSQYPFHVPLLSSQKMVYPGLQEPWLHSMYHGAAFQLTQGDQLSTHTDGIPHLVLSPSTVFFGAFALSource
(BTI-Tn-5B1-4)High-5InsectCells*Reactivity
n/aTestedApplication
n/aStorage
ThelyophilizedTNFbetarecombinantproteinisstableforatleast2yearsfromdateofreceiptat-20ËšC.ReconstitutedTNFbetaisstableforatleast3monthswhenstoredinworkingaliquotswithacarrierproteinat-20ËšC.Aswithanyprotein,exposingTNFbetarecombinantproteintorepeatedfreeze/thawcyclesisnotrecommended.Whenworkingwithproteinscareshouldbetakentokeeprecombinantproteinatacoolandstabletemperature.SpeciesReactivity
n/aPurity
Greaterthan98%bySDS-PAGEgelandHPLCanalyses.
Endotoxinlevelislessthan0.1ngperμg(1EU/μg).