Background
ThethreemammalianisoformsofTGF-β,TGF-β1,β2,β3,signalthroughthesamereceptorandelicitsimilarBIOLOGicalresponses.Theyaremultifunctionalcytokinesthatregulatecellproliferation,growth,differentiationandmotilityaswellassynthesisanddepositionoftheextracellularmatrix.Theyareinvolvedinvariousphysiologicalprocessesincludingembryogenesis,tissueremodelingandwouldhealing.Theyaresecretedpredominantlyaslatentcomplexeswhicharestoredatthecellsurfaceandintheextracellularmatrix.ThereleaseofbiologicallyactiveTGF-βisoformfromalatentcomplexinvolvesproteolyticprocessingofthecomplexand/orinductionofconformationalchangesbyproteinssuchasthrombospondin-1.ThephysiologicalroleofTGF-β3isstillunknownbutitsexpressionpatternsuggestsaroleintheregulationofcertaindevelopmentprocesses.RecombinantTGF-β3isa25.0kDaproteincomposedoftwoidentical112aminoacidpolypeptidechainslinkedbyasingledisulfidebond.Manufacturedusingallnon-animalreagents.
Specifications
AdditionalNames
TransformingGrowthFactor-beta3TestedApplication
n/aDisclaimer
Thisproductisforresearchuseonly.Purity
Greaterthan98%bySDS-PAGEgelandHPLCanalyses.Storage
ThelyophilizedTGF-Beta3recombinantproteinisstableforatleast2yearsfromdateofreceiptat-20ËšC.ReconstitutedTGF-Beta3stableforatleast3monthswhenstoredinworkingaliquotswithacarrierproteinat-20ËšC.Aswithanyprotein,exposingTGF-Beta3recombinantproteintorepeatedfreeze/thawcyclesisnotrecommended.Whenworkingwithproteinscareshouldbetakentokeeprecombinantproteinatacoolandstabletemperature.Preparation
BiologicalactivitywasdeterminedbyTGF-β3¡¯sABIlitytoinhibitthemouseIL-4-dependentproliferationofmouseHT-2cells.TheexpectedED50is¡Ü0.05ng/mL,correspondingtoaspecificactivityof¡Ý2x107units/mg.Endotoxinlevelislessthan0.1ngperμgofTGF-β3(1EU/μg).Reactivity
n/aSource
E.coliLocation
ALDTNYCFRNLEENCCVRPLYIDFRQDLGWKWVHEPKGYYANFCSGPCPYLRSADTTHSTVLGLYNTLNPEASASPCCVPQDLEPLTILYYVGRTPKVEQLSNMVVKSCKCSSpeciesReactivity
n/a