Background
ThethreemammalianformsofTGF-β,TGF-β1,β2,β3,signalthroughthesamereceptorandelicitsimilarBIOLOGicalresponses.Theyaremultifunctionalcytokinesthatregulatecellproliferation,growth,differentiation,andmotilityaswellassyntehsisanddepositionoftheextracellularmatrix.Theyareinvolvedinvariousphysiologicalprocessesinclidingembryogenesis,tissueremodelingandwoundhealing.Theyaresecretedpredominantlyaslatentcomplexeswhicharestoredatthecellsurfaceandintheextracellularmatrix.ThereleaseofbiologicallyactiveTGF-βisoformfromalatentcomplexinvolvesproteolyticprocessingofthecomplexand/orinductionofconformationalchangesbyproteinssuchasthrombospondin-1.ThephysiologicalroleofTGF-β3isstillunknownbutitsexpressionpatternsuggestsaroleintheregulationofcertaindevelopmentprocesses.RecombinantTGF-β3isa25.0kDaproteincomposedoftwoidentical112aminoacidpolypeptidechainslinkedbyasingledisulfidebond.
Specifications
AdditionalNames
TransformingGrowthFactor-beta3Preparation
BiologicalactivitywasdeterminedbyitsABIlitytoinhibitthemouseIL-4-dependentproliferationofmouseHT-2cells.TheED50 wasfoundtobe?0.05ng/ml,correspondingtoaspecificactivityof?2x107 units/mg.Location
ALDTNYCFRNLEENCCVRPLYIDFRQDLGWKWVHEPKGYYANFCSGPCPYLRSADTTHSTVLGLYNTLNPEASASPCCVPQDLEPLTILYYVGRTPKVEQLSNMVVKSCKCSSource
Sf-9cellsReactivity
n/aTestedApplication
n/aStorage
ThelyophilizedTGFbeta3recombinantproteinisstableforatleast2yearsfromdateofreceiptat-20ËšC.ReconstitutedTGFbeta3isstableforatleast3monthswhenstoredinworkingaliquotswithacarrierproteinat-20ËšC.Aswithanyprotein,exposingTGFbeta3recombinantproteintorepeatedfreeze/thawcyclesisnotrecommended.Whenworkingwithproteinscareshouldbetakentokeeprecombinantproteinatacoolandstabletemperature.SpeciesReactivity
n/aPurity
Greaterthan98%bySDS-PAGEgelandHPLCanalyses.
Endotoxinlevelislessthan0.1ngperμg(1EU/μg).