Background
RANKLandRANKaremembersoftheTNFsuperfamilyofligandsandreceptorsthatplayanimportantroleintheregulationofspecificimmunityandboneturnover.RANK(receptor)wasoriginallyidentifiedasadendritic-cell-membraneprotein,whichbyinteractingwithRANKLaugmentstheABIlityofdendriticcellstostimulatenaïveT-cellproliferationinamixedlymphocytereaction,topromotethesurvivalofRANK+Tcells,andtoregulateT-cell-dependentimmuneresponse.RANKL,whichisexpressedinavarietyofcellsincludingosteoblasts,fibroblasts,activatedT-cellsandbonemarrowstromalcells,isalsocapableofinteractingwithadecoyreceptorcalledOPG.BindingofsolubleOPGtosRANKLinhibitsosteoclastogenesisbyinterruptingthesignalingbetweenstromalcellsandosteoclasticProgenitorcells,therebyleADIngtoexcessaccumulationofboneandcartilage.RecombinantratsRANKLisa19.4kDapolypeptidecomprisingtheTNFhomologousregionofRANKL(174aminoacidresidues).
Specifications
AdditionalNames
solubleReceptorActivatorofNFkBLigand,TNFSF11,TRANCE(TNF-relatedactivation-inducedcytokine),OPGL,ODF(Osteoclastdifferentiationfactor)Preparation
BIOLOGicalactivitywasdeterminedbyitsabilitytoinduceosteoclastformationinRAW264.7cellsusingaconcentrationrangeof5.0-10.0ng/ml.Location
PAMMEGSWLDVARRGKPEAQPFAHLTINAADIPSGSHKVSLSSWYHDRGWAKISNMTLSNGKLRVNQDGFYYLYANICFRHHETSGSVPADYLQLMVYVVKTSIKIPSSHNLMKGGSTKNWSGNSEFHFYSINVGGFFKLRAGEEISVQVSNPSLLDPDQDATYFGAFKVQDIDSource
E.coliReactivity
n/aTestedApplication
n/aStorage
ThelyophilizedSolubleRANKLigandrecombinantproteinisstableforatleast2yearsfromdateofreceiptat-20ËšC.ReconstitutedSolubleRANKLigandisstableforatleast3monthswhenstoredinworkingaliquotswithacarrierproteinat-20ËšC.Aswithanyprotein,exposingSolubleRANKLigandrecombinantproteintorepeatedfreeze/thawcyclesisnotrecommended.Whenworkingwithproteinscareshouldbetakentokeeprecombinantproteinatacoolandstabletemperature.SpeciesReactivity
n/aPurity
Greaterthan98%bySDS-PAGEgelandHPLCanalyses.
Endotoxinlevelislessthan0.1ngperμg(1EU/μg).