Background
Slit2 is a member of the Slit family that signals through the Roundabout (Robo) receptor as a repellent for axon guidance and neuronal migration, and can also act as a chemoattractant to vascular endothelial cells and a chemotaxis inhibitor for leukocytes. Slit2 is expressed primarily in the fetal lung, kidney, and adult spinal cord, and to a lesser extent in adult adrenal gland, thyroid and trachea. Slit2 is initially synthesized as a 1499 amino acid precursor, which is subsequently cleaved into N-terminal and C-terminal fragments, designated as Slit2-N and Slit2-C respectively. The neurodevelopment related activities, as measured by the ability to repel olfactory bulb axons and to induce branching in dorsal root ganglia axons, are contained only in the N-terminal fragment. Recombinant human Slit2-N is a 1088 amino acid glycoprotein corresponding to the N-terminal portion of the full length Slit2 precursor. Due to glycosylation Slit2-N migrates at an apparent molecular weight of approximately 120.0-140.0 kDa by SDS-PAGE analysis under reducing conditions.
Specifications
Additional Names
Slit homolog 2 protein, SLIL3
Location
ACPAQCSCSG STVDCHGLAL RSVPRNIPRN TERLDLNGNN ITRITKTDFA GLRHLRVLQL MENKISTIER GAFQDLKELE RLRLNRNHLQ LFPELLFLGT AKLYRLDLSE NQIQAIPRKA FRGAVDIKNL QLDYNQISCI EDGAFRALRD LEVLTLNNNN ITRLSVASFN HMPKLRTFRL HSNNLYCDCH LAWLSDWLRQ RPRVGLYTQC MGPSHLRGHN VAEVQKREFV CSGHQSFMAP SCSVLHCPAA CTCSNNIVDC RGKGLTEIPT NLPETITEIR LEQNTIKVIP PGAFSPYKKL RRIDLSNNQI SELAPDAFQG LRSLNSLVLY GNKITELPKS LFEGLFSLQL LLLNANKINC LRVDAFQDLH NLNLLSLYDN KLQTIAKGTF SPLRAIQTMH LAQNPFICDC HLKWLADYLH TNPIETSGAR CTSPRRLANK RIGQIKSKKF RCSAKEQYFI PGTEDYRSKL SGDCFADLAC PEKCRCEGTT VDCSNQKLNK IPEHIPQYTA ELRLNNNEFT VLEATGIFKK LPQLRKINFS NNKITDIEEG AFEGASGVNE ILLTSNRLEN VQHKMFKGLE SLKTLMLRSN RITCVGNDSF IGLSSVRLLS LYDNQITTVA PGAFDTLHSL STLNLLANPF NCNCYLAWLG EWLRKKRIVT GNPRCQKPYF LKEIPIQDVA IQDFTCDDGN DDNSCSPLSR CPTECTCLDT VVRCSNKGLK VLPKGIPRDV TELYLDGNQF TLVPKELSNY KHLTLIDLSN NRISTLSNQS FSNMTQLLTL ILSYNRLRCI PPRTFDGLKS LRLLSLHGND ISVVPEGAFN DLSALSHLAI GANPLYCDCN MQWLSDWVKS EYKEPGIARC AGPGEMADKL LLTTPSKKFT CQGPVDVNIL AKCNPCLSNP CKNDGTCNSD PVDFYRCTCP YGFKGQDCDV PIHACISNPC KHGGTCHLKE GEEDGFWCIC ADGFEGENCE VNVDDCEDND CENNSTCVDG INNYTCLCPP EYTGELCEEK LDFCAQDLNP CQHDSKCILT PKGFKCDCTP GYVGEHCDID FDDCQDNKCK NGAHCTDAVN GYTCICPEGY SGLFCEFSPP MV
Source
293 cells
Reactivity
n/a
Tested Application
n/a
Storage
The lyophilized Slit2 recombinant protein is stable for at least 2 years from date of receipt at -20ËšC. Reconstituted Slit2 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20ËšC. As with any protein, exposing Slit2 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Species Reactivity
n/a
Purity
Greater than 98% by SDS-PAGE gel and HPLC analyses.
Endotoxin level is less than 0.1 ng per μg (1EU/μg).
