Background
CD30 ligand (CD30L) is a type-II membrane-associated glycoprotein protein belonging to the TNF superfamily and is expressed primarily on certain B cells, T cells, and monocytes. CD30L binds specifically to CD30 (receptor), which is expressed on activated, but not resting, B and T cells, in lymphomas and various chronically inflamed tissues. CD30L/CD30 interactions initiate a signaling cascade that can ultimately lead to the activation of NF-KappaB. CD30L/CD30 signaling exerts pleiotropic effects on normal cells, including cell death, differentiation, or cell division. Certain diseases, including Hodgkins¡¯s lymphoma, allergic inflammation, diabetes (in NOD mice), and mycobacterial infection can also be affected by CD30L/CD30 signaling. The CD30L gene encodes for a 234 amino acid type II transmembrane protein, which contains a 37 amino acid cytoplasmic sequence, a 25 amino acid transmembrane domain and a 172 amino acid extracellular domain. Recombinant human soluble CD30L (sCD30L) is a 188 amino acid polypeptide corresponding to the extracellular domain, and contains an 8 residue N-terminal His -Tag.
Specifications
Additional Names
TNFSF8, CD153, CD30L
Tested Application
n/a
Disclaimer
This product is for research use only.
Purity
Greater than 95% by SDS-PAGE gel and HPLC analyses.
Storage
The lyophilized sCD30 Ligand recombinant protein is stable for at least 2 years from date of receipt at -20ËšC. Reconstituted sCD30 Ligand stable for at least 3 months when stored in working aliquots with a carrier protein at -20ËšC. As with any protein, exposing sCD30 Ligand recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Preparation
Biological activity was determined by its ability to stimulate human IL-8 production by human PBMC using a concentration range of 10.0-25.0 ng/mL. Note: Results may vary with PBMC donors. Endotoxin level is less than 0.1 ng per μg (1EU/μg).
Reactivity
n/a
Source
CHO cells
Location
HHHHHHHHPS PGGSGGQRTD SIPNSPDNVP LKGGNCSEDL LCILKRAPFK KSWAYLQVAK HLNKTKLSWN KDGILHGVRY QDGNLVIQFP GLYFIICQLQ FLVQCPNNSV DLKLELLINK HIKKQALVTV CESGMQTKHV YQNLSQFLLD YLQVNTTISV NVDTFQYIDT STFPLENVLS IFLYSNSD
Species Reactivity
n/a