Background
CD27 Ligand, a type II transmembrane protein, is a member of the TNF superfamily. It is expressed on activated T and B lymphocytes as well as NK cells. CD27L and its receptor (CD27) regulate the immune response by promoting T-cell expansion and differentiation, as well as NK enhancement. CD27 signaling can act as a co-stimulatory effector to sustain the survival of CD8+ T cells, primarily by inducing increased expression of the IL-2 gene. Full length human CD27L is a 193 amino acid protein, consisting of a 17 amino acid cytoplasmic domain, a 21 amino acid transmembrane domain, and a 155 amino acid extracellular domain. Human soluble CD27L corresponds to the 155 amino acid extracellular domain of the full length CD27L protein. ProSci¡¯s recombinant human sCD27L contains the extracellular domain plus an N-terminal His-Tag.
Specifications
Additional Names
TNFSF7, CD70
Tested Application
n/a
Disclaimer
This product is for research use only.
Purity
Greater than 95% by SDS-PAGE gel and HPLC analyses.
Storage
The lyophilized sCD27 Ligand recombinant protein is stable for at least 2 years from date of receipt at -20ËšC. Reconstituted sCD27 Ligand stable for at least 3 months when stored in working aliquots with a carrier protein at -20ËšC. As with any protein, exposing sCD27 Ligand recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Preparation
Biological activity was determined by its ability to stimulate human IL-8 production by human PBMC using a concentration range of 10.0-25.0 ng/mL. Note: Results may vary with PBMC donors. Endotoxin level is less than 0.1 ng per μg (1EU/μg).
Reactivity
n/a
Source
CHO cells
Location
HHHHHHHHPS PGGSGGQRFA QAQQQLPLES LGWDVAELQL NHTGPQQDPR LYWQGGPALG RSFLHGPELD KGQLRIHRDG IYMVHIQVTL AICSSTTASR HHPTTLAVGI CSPASRSISL LRLSFHQGCT IASQRLTPLA RGDTLCTNLT GTLLPSRNTD ETFFGVQWVR P
Species Reactivity
n/a
