Background
PECAMistransmembraneglycoproteinthatbelongstotheIg-relatedsuperfamilyofadhesionmolecules.Itishighlyexpressedatendothelialcelljunctions,andalsoexpressedinplateletsandinmostleukocytesub-types.TheprimaryfunctionofPECAM-1isthemediationofleukocyte-endothelialcelladhesionandsignaltransduction.PECAM-1hasbeenimplicatedinthepathogenesisofvariousinflammationrelateddisorders,includingthrombosis,multiplesclerosis(MS),andrheumatoidarthritis.ThehumanPECAM-1genecodesfora738aminoacidtransmembraneglycoproteincontaininga118aminoacidcytoplasmicdomain,a19aminoacidtransmembranedomain,anda574aminoacidextracellulardomain.RecombinanthumanPECAM-1isa572aminoacidglycoproteincomprisingtheextracellulardomainofPECAM-1.MonomericglycosylatedPECAM-1migratesatanapparentmolecularweightofapproximately80.0-95.0kDabySDS-PAGEanalysisunderreducingconditions
Specifications
Location
ENSFTINSVDMKSLPDWTVQNGKNLTLQCFADVSTTSHVKPQHQMLFYKDDVLFYNISSMKSTESYFIPEVRIYDSGTYKCTVIVNNKEKTTAEYQLLVEGVPSPRVTLDKKEAIQGGIVRVNCSVPEEKAPIHFTIEKLELNEKMVKLKREKNSRDQNFVILEFPVEEQDRVLSFRCQARIISGIHMQTSESTKSELVTVTESFSTPKFHISPTGMIMEGAQLHIKCTIQVTHLAQEFPEIIIQKDKAIVAHNRHGNKAVYSVMAMVEHSGNYTCKVESSRISKVSSIVVNITELFSKPELESSFTHLDQGERLNLSCSIPGAPPANFTIQKEDTIVSQTQDFTKIASKSDSGTYICTAGIDKVVKKSNTVQIVVCEMLSQPRISYDAQFEVIKGQTIEVRCESISGTLPISYQLLKTSKVLENSTKNSNDPAVFKDNPTEDVEYQCVADNCHSHAKMLSEVLRVKVIAPVDEVQISILSSKVVESGEDIVLQCAVNEGSGPITYKFYREKEGKPFYQMTSNATQAFWTKQKASKEQEGEYYCTAFNRANHASSVPRSKILTVRVILAPWKSource
HEK293cellsReactivity
n/aPreparation
Endotoxinlevelislessthan0.1ngperug(1EU/μg).Storage
ThelyophilizedPECAM-1recombinantproteinisstableforatleast2yearsfromdateofreceiptat-20ËšC.ReconstitutedPECAM-1isstableforatleast3monthswhenstoredinworkingaliquotswithacarrierproteinat-20ËšC.Aswithanyprotein,exposingPECAM-1recombinantproteintorepeatedfreeze/thawcyclesisnotrecommended.Whenworkingwithproteinscareshouldbetakentokeeprecombinantproteinatacoolandstabletemperature.Purity
Greaterthanorequalto97%bySDS-PAGEgelandHPLCanalyses.Disclaimer
Thisproductisforresearchuseonly.TestedApplication
n/aSpeciesReactivity
n/a