Background
PDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs; PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet α-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-α and PDGFR-B. PDGFR-a is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-B interacts with only PDGF-BB and PDGF-AB. Recombinant human PDGF-AB is a 25.5 kDa disulfide-linked dimer, consisting of one A chain and one B chains (234 total amino acids).
Specifications
Location
Alpha Chain MSIEEAVPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATTSLN PDYREEDTGR PRESGKKRKR KRLKPT
Beta Chain MSLGSLTIAE PAMIAECKTR TEVFEISRRL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRPTQVQLRP VQVRKIGIVR KKPIFKKATV TLGDHLACKC ETVAAARPVT
Source
E. coli
Reactivity
n/a
Preparation
The ED50 was determined by the dose-dependent stimulation of thymidine uptake by BALB/c 3T3 cells is ¡Ü 1 ng/mL, corresponding to a specific activity of ¡Ý 1 x 106 units/mg.
Storage
The lyophilized PDGF-AB recombinant protein is stable for at least 2 years from date of receipt at -20ËšC. Reconstituted PDGF-AB is stable for at least 3 months when stored in working aliquots with a carrier protein at -20ËšC. As with any protein, exposing PDGF-AB recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Purity
Greater than 98% by SDS-PAGE gel and HPLC analyses.
Endotoxin level is less than 0.1 ng per μg (1EU/μg).
Disclaimer
This product is for research use only.
Tested Application
n/a
Species Reactivity
n/a
