Background
Oncostatin M (OSM) is a growth and differentiation factor that participates in the regulation of neurogenesis, osteogenesis and hematopoiesis. Produced by activated T cells, monocytes and Kaposi¡¯s sarcoma cells, OSM can exert both stimulatory and inhibitory effects on cell proliferation. It stimulates the proliferation of fibroblasts, smooth muscle cells and Kaposi¡¯s sarcoma cells, but, inhibits the growth of some normal and tumor cell lines. It also promotes cytokine release (e.g. IL-6, GM-CSF and G-CSF) from endothelial cells, and enhances the expression of low-density lipoprotein receptor in hepatoma cells. OSM share several structural and functional characteristics with LIF, IL-6, and CNTF. Human OSM is active on murine cells. Recombinant rat Oncostatin M is a 24.4 kDa protein, containing 215 amino acid residues.
Specifications
Additional Names
OSM
Tested Application
n/a
Disclaimer
This product is for research use only.
Purity
Greater than 98% by SDS-PAGE gel and HPLC analyses.
Storage
The lyophilized Oncostatin M recombinant protein is stable for at least 2 years from date of receipt at -20ËšC. Reconstituted Oncostatin M stable for at least 3 months when stored in working aliquots with a carrier protein at -20ËšC. As with any protein, exposing Oncostatin M recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Preparation
Assay 1: Biological activity was determined by its ability to stimulate the proliferation of rat C6 cells. The expected ED50 is 3.0-5.0 μg/mL.
Assay 2: Biological activity was determined by its ability to inhibit Alkaline phosphatase activity of differentiated MC3T3 E1 cells. Endotoxin level is less than 0.1 ng per μg (1EU/μg).
Reactivity
n/a
Source
E.coli
Location
MKRGCSSSSP KLLSQLKSQA NITGNTASLL EPYILHQNLN TLTLRAACTE HPVAFPSEDM LRQLSKPDFL STVHATLGRV WHQLGAFRQQ FPKIQDFPEL ERARQNIQGI RNNVYCMARL LHPPLEIPEP TQADSGTSRP TTTAPGIFQI KIDSCRFLWG YHRFMGSVGR VFEEWGDGSR RSRRHSPLWA WLKGDHRIRP SRSSQSAMLR SLVPR
Species Reactivity
n/a
