Background
Neurturinisadisulfide-linkedhomodimerneurotrophicfactorstructurallyrelatedtoGDNF,Artemin,andPersephin.Theseproteinsbelongtothecysteine-knotfamilyofgrowthfactorsthatassumestabledimericstructures.Neurturinsignalsthroughamulticomponentreceptorsystem,composedofRETandoneoffourGFRalpha(alpha1-alpha4)receptors.NeurturinpromotesthedevelopmentandsurvivalofsympatheticandsensoryneuronsbysignalingthroughareceptorsystemcomposedofRETandGFRalpha2.ThefunctionalformofhumanNeurturinisadisulfide-linkedhomodimer,oftwo11.8kDapolypeptidemonomers(206totalaminoacidresidues).Eachmonomercontainssevenconservedcysteineresidues,oneofwhich(Cys69)isusedforinter-chaindisulfidebridgingandtheothersareinvolvedinintramolecularringformationknownasthecysteineknotconfiguration.
Specifications
AdditionalNames
NTN,NRTNPreparation
HumanNeurturinataconcentrationof100ng/mlwillsupportthesurvivalof65%ofnewbornratsympatheticneurons.Location
MARLGARPCGLRELEVRVSELGLGYASDETVLFRYCAGACEAAARVYDLGLRRLRQRRRLRRERVRAQPCCRPTAYEDEVSFLDAHSRYHTVHELSARECACVSource
E.coliReactivity
n/aTestedApplication
n/aStorage
ThelyophilizedNeurturinrecombinantproteinisstableforatleast2yearsfromdateofreceiptat-20ËšC.ReconstitutedNeurturinisstableforatleast3monthswhenstoredinworkingaliquotswithacarrierproteinat-20ËšC.Aswithanyprotein,exposingNeurturinrecombinantproteintorepeatedfreeze/thawcyclesisnotrecommended.Whenworkingwithproteinscareshouldbetakentokeeprecombinantproteinatacoolandstabletemperature.SpeciesReactivity
n/aPurity
Greaterthan98%bySDS-PAGEgelandHPLCanalyses.
Endotoxinlevelislessthan0.1ngperμg(1EU/μg).