Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. The Bcl-2 family of proteins is comprised of critical regulators of apoptosis that can be divided into two classes: those that inhibit apoptosis and those that promote cell death. MTCH2 is a member of the mitochondrial carrier protein family that catalyze the exchange of substrates across the inner mitochondrial membrane and is targeted by Bid, a pro-apoptotic Bcl-2 family member, in response to apoptotic signals, suggesting that MTCH2 may play a key role in the mitochondrial apoptosis pathway. The doublet observed in the SDS-PAGE is likely due to incomplete removal of the signal sequence.
SDS-PAGE analysis of recombinant MTCH2 on Coomassie Blue-stained 12% SDS-PAGE gel.
Protein Accession Number
P27656
Application
This recombinant protein can be used for WB, ELISA, MS and neutralization assays.
Buffer
1X PBS containing 0.1% SDS
Fusion Partner
C-terminal His-tag
Molecular Weight
19 kDa (calculated)
Protein GI Number
5815347
Source
E. coli
Tested Application
E, WB, MS
Storage
Store at -70ËšC. As with any protein, exposing MTCH2 recombinant protein to repeated freeze/thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Species Reactivity
n/a
Purity
~95%
Reference
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Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7.
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Cory S, Huang DCS, and Adams JM. The Bcl-2 family: roles in cell survival and oncogenesis. Oncogene 2003; 22:8590-607.
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Heiser D, Labi V, Erlacher M, et al. The Bcl-2 protein family and its role in the development of neoplastic disease. Exp. Geron. 2004; 39:1125-35.
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Grinberg M, Schwarz M, Zaltsman Y, et al. Mitochondrial carrier homolog 2 is a target of tBID in cells signaled to die by tumor necrosis factor alpha. Mol. Cell. Biol. 2005; 25:4579-90.]
