Background
Klothoisaglycosylatedproteinthatplaysanimportantroleintheregulationofphosphateandcalciumhomeostasis.ThefulllengthtransmembraneformhasalargeextracellulardomaincomposedoftwohomologoussubunitstermedKL1andKL2,whichcontain516and439aminoacidresidues,respectively,Thepredominantcirculatingform,whichisderivedfromalternativeRNAsplicing,containstheKL1subunitandconstitutestheN-terminalsequenceoftransmembraneKlotho.AthirdKlothoproteinofabout128kDahasbeenidentifiedinthebloodandcerebrospinalfluid.ThiscirculatingproteinarisesfromtheactionofanasyetunidentifiedproteasewhichcleavestransmembraneKlothojustaboveand/orwithintheplasmamembrane.Klothohasbeenshowntoplayakeyroleinthesignalingcascadeoffibroblastgrowthfactor-23(FGF-23),abonederivedhormonethatactsinthekidneytoinhibitphosphatereabsorptionandvitaminDbiosynthesis.KlothopromotesFGF-23signalingthroughbindingtoFGFRI(IIIc)whichconvertsthiscanonicalFGFreceptorintoaspecificreceptorforFGF-23.IntheabsenceofKlothothefunctionofFGF-23isliterallyabolished.RecombinanthumanKlothoisa65-70kDaglycoproteincontaining516aminoacidresidues.
Specifications
AdditionalNames
?Klotho,KLAPreparation
BIOLOGicalactivitywasdeterminedbythedose-dependentstimulationoftheproliferationofmurineNIH-3T3cells.RecombinanthumanKlothoiseffectiveinaconcentrationrangeof0.5-2.0μg/ml.Location
EPGDGAQTWARFSRPPAPEAAGLFQGTFPDGFLWAVGSAAYQTEGGWQQHGKGASIWDTFTHHPLAPPGDSRNASLPLGAPSPLQPATGDVASDSYNNVFRDTEALRELGVTHYRFSISWARVLPNGSAGVPNREGLRYYRRLLERLRELGVQPVVTLYHWDLPQRLQDAYGGWANRALADHFRDYAELCFRHFGGQVKYWITIDNPYVVAWHGYATGRLAPGIRGSPRLGYLVAHNLLLAHAKVWHLYNTSFRPTQGGQVSIALSSHWINPRRMTDHSIKECQKSLDFVLGWFAKPVFIDGDYPESMKNNLSSILPDFTESEKKFIKGTADFFALCFGPTLSFQLLDPHMKFRQLESPNLRQLLSWIDLEFNHPQIFIVENGWFVSGTTKRDDAKYMYYLKKFIMETLKAIKLDGVDVIGYTAWSLMDGFEWHRGYSIRRGLFYVDFLSQDKMLLPKSSALFYQKLIEKNGFPPLPENQPLEGTFPCDFAWGVVDNYIQVSQLTKPISSLTKPYHSource
CHOcells(ChineseHamsterOvariancells)Reactivity
n/aTestedApplication
n/aStorage
ThelyophilizedKlothorecombinantproteinisstableforatleast2yearsfromdateofreceiptat-20ËšC.ReconstitutedKlothoisstableforatleast3monthswhenstoredinworkingaliquotswithacarrierproteinat-20ËšC.Aswithanyprotein,exposingKlothorecombinantproteintorepeatedfreeze/thawcyclesisnotrecommended.Whenworkingwithproteinscareshouldbetakentokeeprecombinantproteinatacoolandstabletemperature.SpeciesReactivity
n/aPurity
Greaterthan98%bySDS-PAGEgelandHPLCanalyses.
Endotoxinlevelislessthan0.1ngperμg(1EU/μg).