Background
IL-21 is a pleiotropic cytokine produced by CD4+ T cells in response to antigenic stimulation. Its action generally enhances antigen-specific responses of immune cells. The biological effects of IL-21 include induction of differentiation of T-cells-stimulated B-cells into plasma cells and memory B-cells, stimulation (in conjunction) with IL-4 of IgG production, and induction of apoptotic effects in naive B-cells and stimulated B-cells in the absence of T-cell signaling. Additionally, IL-21 promotes the anti-tumor activity of CD8+ T-cells and NK cells. IL-21 exerts its effect through binding to a specific type I cytokine receptor, IL-21R, which also contains the gamma chain (γc) found in other cytokine receptors including IL-2, IL-4, IL-7, IL-9 and IL-15. The IL-21/IL-21R interaction triggers a cascade of events which includes activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3. Recombinant human IL-21 is a 15.0 kDa protein consisting of 133 amino acid residues. Manufactured using all non-animal reagents.
Specifications
Location
MQDRHMIRMR QLIDIVDQLK NYVNDLVPEF LPAPEDVETN CEWSAFSCFQ KAQLKSANTG NNERIINVSI KKLKRKPPST NAGRRQKHRL TCPSCDSYEK KPPKEFLERF KSLLQKMIHQ HLSSRTHGSE DS
Purity
Greater than 98% by SDS-PAGE gel and HPLC analyses.
Endotoxin level is less than 0.1 ng per μg (1EU/μg).
Species Reactivity
n/a
Reference
-
Avery, D.T. et. al. (2008) J. Immunol. 181, 1767-1779.]
Storage
The lyophilized IL-21 recombinant protein is stable for at least 2 years from date of receipt at -20ËšC. Reconstituted IL-21 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20ËšC. As with any protein, exposing IL-21 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Tested Application
n/a
Preparation
Assay 1: Determined by its ability to proliferate activated B cells.
Assay 2: Determined by its ability to induce human CD40L-activated naive B cells to undergo Ig isotype switching to IgG, using an IL-21 concentration of 50 ng/mL. Maximal activity was achieved after approximately five cell divisions
Reactivity
n/a
Source
E. coli
Disclaimer
This product is for research use only.
