IL-16 was initially identified as a chemotactic cytokine (1), but is now known to possess a wide range of activities. Later studies have more fully characterized IL-16 as an immunomodulatory cytokine that contributes to the regulatory process of CD4+ T cell recruitment and activation at sites of inflammation in association with asthma and several autoimmune diseases (2). The precursor of IL-16 (pro-IL-16) is thought to be cleaved towards the C-terminal region by Caspase-3, releasing a 20 kDa active form that binds to and signals through CD4 (3,4). Besides acting as a chemotactic cytokine, IL-16 is thought to also be involved in the regulation of T cell proliferation (5) and multiple infectious, immune-mediated, and autoimmune inflammatory disorders including irritable bowel syndrome, systemic lupus erythematosus, and neurodegenerative disorders (6). At least two isoforms of IL-16 are known to exist; the longer isoform (also known as NIL-16) is detected only in neurons of the cerebellum and hippocampus (7). This recombinant protein fragment is derrived from this longer isoform.
SDS-PAGE analysis of recombinant IL-16 on Coomassie Blue-stained 4 – 12% SDS-PAGE gel.
Additional Names
Neuronal IL-16
Fusion Partner
C-terminal His-tag
Molecular Weight
29 kDa (calculated)
Protein Accession Number
AAQ86961
Protein GI Number
36953836
Buffer
20mM Tris, 0.5M NaCl, 10%glycerol, 250mM Imidazole, 5mM beta-mercaptoethanol
Application
This recombinant protein can be used for WB, ELISA, MS and neutralization assays.
Source
E. coli
Tested Application
E, WB, MS
Storage
Store at -70ËšC. As with any protein, exposing p24 recombinant protein to repeated freeze/thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Species Reactivity
n/a
Purity
~95%
Reference
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Cruikshank WW, Center DM, Nisar N, et al. Molecular and functional analysis of a lymphocyte chemoattractant factor: association of biologic function with CD expression.Proc. Natl. Acad. Sci. USA 1994; 91:5109-13.
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Interleukin-16. Cruikshank WW, Kornfeld H, and Center DM. J. Leukoc. Biol. 2000; 67:757-66.
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Zhang Y, Center DM, Wu DM, et al. Processing and activation of pro-interleukin-16 by caspase-3. J. Biol. Chem. 1998; 273:1144-9.
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Maciaszek JW, Parada NA, Cruikshank WW, et al. IL-16 represses HIV-1 promoter activity.J. Immunol. 1997; 158:5-8. ]
