Background
GDF-5 is expressed in long bones during embryonic development and postnatally in articular cartilage. Mutations in the GDF-5 gene have been implicated in Grebe Syndrome, which is characterized by short stature, extra digits, short and deformed extremities, and in Hunter-Thompson type dwarfism. The mature and functional form of GDF-5 is a homodimer of two 120 amino-acid polypeptide chain (monomers) linked by a single disulfide bond. Each GDF-5 monomer is expressed as the C-terminal part of a precursor polypeptide, which also contains a 27 amino-acid signal peptide and a 348 amino-acid propeptide. This precursor undergoes intracellular dimerization, and upon secretion it is processed by a furin-type protease. Recombinant murine GDF-5 is a 27.0 kDa homodimeric disulfide-linked protein consisting of two 120 amino acids.
Specifications
Additional Names
BMP-14, Cartilage-Derived Morphogenetic Protein-1 (CDMP-1)
Purity
Greater than 98% by SDS-PAGE gel and HPLC analyses.
Species Reactivity
n/a
Storage
The lyophilized GDF-5 recombinant protein is stable for at least 2 years from date of receipt at -20ËšC. Reconstituted GDF-5 stable for at least 3 months when stored in working aliquots with a carrier protein at -20ËšC. As with any protein, exposing GDF-5 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Tested Application
n/a
Preparation
Endotoxin level is less than 0.1 ng per μg (1EU/μg).
Reactivity
n/a
Source
E.coli
Location
APLANRQGKR PSKNLKARCS RKALHVNFKD MGWDDWIIAP LEYEAFHCEG LCEFPLRSHL EPTNHAVIQT LMNSMDPEST PPTCCVPTRL SPISILFIDS ANNVVYKQYE DMVVESCGCR
Disclaimer
This product is for research use only.
