The integrin alpha 4 (also known as CD49d and ITGA4) belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha and beta chains. Alpha 4 chain associates with either beta 1 or beta 7 chain. It has been demonstrated that the putative ligand-binding sites of both integrin alpha 4 beta 1 and alpha 4 beta 7 is located on the alpha 4 chain. These ligands included Madcam, VCAM, and fibronectin. Madcam is known as the principal ligand for integrin alpha 4 beta 7. Recently it was also demonstrated that HIV-1 envelope can mimic Madcam by binding to and signaling though integrin alpha 4 beta 7, the gut mucosal homing receptor for peripheral T cells. This recombinant protein fragment includes the ligand and monoclonal antibody binding domains; this binding has been tested and verified in ELISA assays. This recombinant protein is recognized PSI antibodies Integrin 4 alpha (IN1) (PSI 4827) and Integrin 4 alpha (IN2) (PSI 4837), but not Integrin 4 alpha (CT) (PSI 4783).
SDS-PAGE analysis of recombinant Integrin alpha 4 fragment on Coomassie Blue-stained 4-20% gradient gel.
Additional Names
CD49d, ITGA4
Fusion Partner
N-terminal His-tag
Molecular Weight
17 kDa (calculated)
Protein Accession Number
NP_000876
Protein GI Number
67191027
Buffer
50mM Tris pH7.5, 150mM NaCl, 5 mM MgCl2
Application
This recombinant protein can be used for WB, ELISA, MS and neutralization assays.
Source
E. coli
Tested Application
E, WB, MS
Storage
Store at -70ËšC. As with any protein, exposing Integrin alpha 4 recombinant protein to repeated freeze/thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Species Reactivity
n/a
Purity
~95%
Reference
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Wegener KL and Campbell ID. Transmembrane and cytoplasmic domains in integrin activation and protein-protein interactions. Mol. Membr. Biol. 2008; 25:376-87.
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Kamata T, Puzon W, and Takada Y. Identification of putative ligand-binding sites of the integrin alpha 4 beta 1 (VLA-4, CD49d/CD29). Biochem J. 1995; 305:945-51.
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Berlin C, Berg EL, Briskin MJ, et al. Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1. Cell 1993; 74:185-95.
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Pulido R, Elices MJ, Campanero MR, et al. Functional evidence for three distinct and independently inhibitable adhesion activities mediated by the human integrin VLA-4. Correlation with distinct alpha 4 epitopes. J. Biol. Chem. 1991; 266:10241-5.]
