>
产品中心 >
Other_kits >
Zyagen/Panel 4: Mouse C57 Digestive Tissue Premade Northern Blot/11411/1 Ea
MouseC57 DiestivetissueNorthernblotismadeoftotalRNAextractedfrom11digestivetissuesof C57BL/6 mousestrain(Panel#4).TotalRNAsamplesaresubjectedtorigorousqualitycontrolprocedures beforefractionationthroughlargedenaturedagarosegels. BlotscanbeusedwitheitherrADIoactiveor nonisotopic-labeledprobesto:
QuicklyassessgeneexpressionacrossC57mousetissues.
Determinesizeandrelativeabundanceoftranscriptsindifferenttissues.
Identifyalternativespliceforms.
Qualitycontrol:
Everystepofpreparationofblots,fromharvestingtissuesandextractionofRNAtotheblotting,iscarefullymonitoredtoensurethesuperiorqualityandperformance.
Blotsaremade oftotalRNAthatistreatedwithRNase-freeDNasetoremoveresidualDNA.ThepurityandintegrityofRNAaretestedbydenaturingagarosegelelectrophoresis.
TheefficiencyoftransferischeckedbystaininggelswithreversIBLestaining.
TheintegrityoftheblottedRNAistestedbybeta-actinspecificprobe.
Description: ThefreshlyisolatedtotalRNAsamples(20ugeach)plusoneRNAMarkerarefractionatedthroughlarge formaldehyde-denaturedagarosegeleletrophoresis,blottedontoapositivelychargednylonmembranes,andimmobilizedbyUVlight.Blotisshippedinasealedbagatroomtemperatureand isgoodfor1yearifproperlystoredat4oCinthesealedbag.