Background
EPAC1, also known as Rap guanine nuclear exchange factor 3 and cAMPGEF-I, is expressed most prominently in brain, heart, kidney, pancreas, spleen, ovary, thyroid and spinal chord. Epac1 is a cAMP-binding protein with intrinsic guanine nuclear exchange factor activity that couples cAMP production to the activation of Rap, a GTPase belonging to the Ras family. This activation of Rap influeces numerous cellular processes such as integrin-mediated cell adhesion, vascular endothelial barrier formation, and cardiac myocyte gap junction formation. Recently, Epac1 has been suggested to also be involved in the cAMP-dependent regulation of ion channel formatioon, intracellular Ca++ signalling, ion transporter activity, and exocytosis.
Description
SDS-PAGE analysis of recombinant EPAC1 on Coomassie Blue-stained 12% SDS-PAGE gel.
Specifications
Protein Accession Number
NP_659099
Application
This recombinant protein can be used for WB, ELISA, MS and neutralization assays.
Buffer
1X PBS containing 0.1% SDS
Fusion Partner
C-terminal His-tag
Molecular Weight
19 kDa (calculated)
Protein GI Number
21450061
Source
E. coli
Tested Application
E, WB, MS
Storage
Store at -70ËšC. As with any protein, exposing EPAC1 recombinant protein to repeated freeze/thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Species Reactivity
n/a
Purity
~95%
Reference
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de Rooij J, Zwartkruis FJ, Verheijen MH, et al. Epac is a Rap1 guanine-nucleotide-exchange factor directly activated by cyclic AMP. Nature 1998; 396:474-7.
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Bos JL. Epac: a new cAMP target and new avenues. Hum. Immunol. 2004; 65:282-90.
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Holz GG, Kang G, Harbeck M, et al. Cell physiology of cAMP sensor Epac. J. Physiol. 2006; 577:5-15.]
