Background
Proteases(alsocalledProteolyticEnzymes,Peptidases,orProteinases)areenzymesthathydrolyzetheamidebondswithinproteinsorpeptides.Mostproteasesactinaspecificmanner,hydrolyzingbondsatoradjacenttospecificresiduesoraspecificsequenceofresiduescontainedwithinthesubstrateproteinorpeptide.ProteasesplayanimportantroleinmostdiseasesandBIOLOGicalprocessesincludingprenatalandpostnataldevelopment,reproduction,signaltransduction,theimmuneresponse,variousautoimmuneanddegenerativediseases,andcancer.Theyarealsoanimportantresearchtool,frequentlyusedintheanalysisandproductionofproteins.EnterokinasesequentiallycleavescarboxylsideofD-D-D-D-K.HumanEnterokinaseisexpressedasalinear1019aminoacidpolypeptideprecursorglycoprotein.ProteolyticprocessingofthisprecursorgeneratesthebiologicallyactiveformofEnterokinase,whichconsistsoftwopolypeptidechains(heavychainandlightchain)heldtogetherbyasingledisulfidebond,resultinginformationofabiologicallyactiveheterodimer.Theheavychainconsistsof784aminoacidresidues,andthelightconsistsof235aminoacidresidues.
Specifications
Location
Heavychain:LTIKESQRGAALGQSHEARATFKITSGVTYNPNLQDKLSVDFKVLAFDLQQMIDEIFLSSNLKNEYKNSRVLQFENGSIIVVFDLFFAQWVSDQNVKEELIQGLEANKSSQLVTFHIDLNSVDILDKLTTTSHLATPGNVSIECLPGSSPCTDALTCIKADLFCDGEVNCPDGSDEDNKMCATVCDGRFLLTGSSGSFQATHYPKPSETSVVCQWIIRVNQGLSIKLSFDDFNTYYTDILDIYEGVGSSKILRASIWETNPGTIRIFSNQVTATFLIESDESDYVGFNATYTAFNSSELNNYEKINCNFEDGFCFWVQDLNDDNEWERIQGSTFSPFTGPNFDHTFGNASGFYISTPTGPGGRQERVGLLSLPLDPTLEPACLSFWYHMYGENVHKLSINISNDQNMEKTVFQKEGNYGDNWNYGQVTLNETVKFKVAFNAFKNKILSDIALDDISLTYGICNGSLYPEPTLVPTPPPELPTDCGGPFELWEPNTTFSSTNFPNSYPNLAFCVWILNAQKGKNIQLHFQEFDLENINDVVEIRDGEEADSLLLAVYTGPGPVKDVFSTTNRMTVLLITNDVLARGGFKANFTTGYHLGIPEPCKADHFQCKNGECVPLVNLCDGHLHCEDGSDEADCVRFFNGTTNNNGLVRFRIQSIWHTACAENWTTQISNDVCQLLGLGSGNSSKPIFSTDGGPFVKLNTAPDGHLILTPSQQCLQDSLIRLQCNHKSCGKKLAAQDITPKLightChain:IVGGSNAKEGAWPWVVGLYYGGRLLCGASLVSSDWLVSAAHCVYGRNLEPSKWTAILGLHMKSNLTSPQTVPRLIDEIVINPHYNRRRKDNDIAMMHLEFKVNYTDYIQPICLPEENQVFPPGRNCSIAGWGTVVYQGTTANILQEADVPLLSNERCQQQMPEYNITENMICAGYEEGGIDSCQGDSGGPLMCQENNRWFLAGVTSFGYKCALPNRPGVYARVSRFTEWIQSFLHSource
CHOcellsReactivity
n/aPreparation
SequentiallycleavescarboxylsideofD-D-D-D-K.Endotoxinlevelislessthan0.2ngperμg(2EU/μg).Storage
ThelyophilizedEnterokinaserecombinantproteinisstableforatleast2yearsfromdateofreceiptat-20ËšC.ReconstitutedEnterokinasestableforatleast3monthswhenstoredinworkingaliquotswithacarrierproteinat-20ËšC.Aswithanyprotein,exposingEnterokinaserecombinantproteintorepeatedfreeze/thawcyclesisnotrecommended.Whenworkingwithproteinscareshouldbetakentokeeprecombinantproteinatacoolandstabletemperature.Purity
Greaterthan90%bySDS-PAGEgelandHPLCanalyses.Disclaimer
Thisproductisforresearchuseonly.TestedApplication
n/aSpeciesReactivity
n/a