Background
Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNF-R1 and Fas. Several death receptors including DR3, DR4, DR5, and DR6 have been identified. Like TNF-R1, DR6 (also known as TNFRSF21) interacts with death domain containing adapter molecule TRADD. Overexpression of DR6 induces apoptosis and activates NF-kB and JNK. DR6 is widely expressed in human tissues and cell lines. Recently, DR6 was found to interact with an amino-terminal fragment of the Beta-amyloid protein (APP) in neurons, activating a caspase 6-dependent apoptotic event leading to axonal degeneration and pruning.
Description
SDS-PAGE analysis of recombinant DR6 on Coomassie Blue-stained 12% SDS-PAGE gel.
Specifications
Additional Names
DR6 Extracellular Domain
Fusion Partner
C-terminal His-tag
Molecular Weight
36 kDa (calculated)
Protein Accession Number
NP_055267
Protein GI Number
7657039
Buffer
1X PBS containing 10% glycerol
Application
This recombinant protein can be used for WB, ELISA, MS and neutralization assays.
Source
E. coli
Tested Application
E, WB, MS
Storage
Store at -70ËšC. As with any protein, exposing DR6 recombinant protein to repeated freeze/thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Species Reactivity
n/a
Purity
~95%
Reference
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Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7.
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Pan G, Bauer JH, Haridas V, et al. Identification and functional characterization of DR6, a novel death domain-containing TNF receptor. FEBS Lett 1998; 431:351-6.
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Nikolaev A, McLaughlin T, O'Leary DD, et al. APP binds DR6 to trigger axon pruning and neuron death via distinct caspases. Nature 2009; 457:981-9.]
