Background
CTGF is a member of the CCN family of secreted cysteine rich regulatory proteins and is the major mitogenic and chemoattractant protein produced by umbilical vein and vascular endothelial cells. CTGF stimulates the proliferation and differentiation of chondrocytes, induces angiogenesis, promotes cell adhesion of fibroblasts, endothelial, and epithelial cells, and binds to IGF, TGF beta1, and BMP-4. Cell migration and adhesion are signaled through binding to specific cell surface integrins and to heparin sulfate proteoglycans CTGF (98 a.a.), a lower molecular weight isoform containing the C-terminal portion of the full length CTGF protein, exerts full heparin binding, cell adhesion, and mitogenic CTGF activity. Recombinant human CTGF is a 11.2 kDa protein of 98 amino acid residues.
Specifications
Additional Names
Connective Tissue Growth Factor, CCN2, Hypertrophic chondrocyte-specific protein 24 (HCS24)
Preparation
Biological activity was determined by the dose - dependent stimulation of the proliferation of HUVEC cells. The expected ED50 for this effect is 1.0 - 2.0 μg/ml.
Location
MGKKCIRTPK ISKPIKFELS GCTSMKTYRA KFCGVCTDGR CCTPHRTTTL PVEFKCPDGE VMKKNMMFIK TCACHYNCPG DNDIFESLYY RKMYGDMA
Source
E. coli
Reactivity
n/a
Tested Application
n/a
Storage
The lyophilized CTGF recombinant protein is stable for at least 2 years from date of receipt at -20ËšC. Reconstituted CTGF is stable for at least 3 months when stored in working aliquots with a carrier protein at -20ËšC. As with any protein, exposing CTGF recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Species Reactivity
n/a
Purity
Greater than 95% by SDS-PAGE gel and HPLC analyses.
Endotoxin level is less than 0.1 ng per μg (1EU/μg).
