Background
TGF-β family members are key modulators of cell proliferation, differentiation, matrix synthesis, and apoptosis. As implied by their name, BMPs initiate, promote, and regulate the development, growth and remodeling of bone and cartilage. In addition to this role, BMPs are also involved in prenatal development and postnatal growth, remodeling and maintenance of a variety of other tissues and organs. BMP-5 is expressed in the nervous system, lung and liver. It is a known regulator for dendritic growth in sympathetic neurons. BMP-5 is a 454 amino acid precursor protein that is cleaved to release the biologically active C-terminal mature protein. Recombinant human BMP-5 is a 15.6 kDa homodimeric disulfide-linked protein consisting of two identical 138 amino acid chains.
Specifications
Additional Names
Bone Morphogenetic protein 5
Tested Application
n/a
Disclaimer
This product is for research use only.
Purity
Greater than 95% by SDS-PAGE gel and HPLC analyses.
Storage
The lyophilized BMP-5 recombinant protein is stable for at least 2 years from date of receipt at -20ËšC. Reconstituted BMP-5 stable for at least 3 months when stored in working aliquots with a carrier protein at -20ËšC. As with any protein, exposing BMP-5 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Preparation
Biological activity was determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is 1.0-2.0 μg/mL. Endotoxin level is less than 0.1 ng per μg (1EU/μg).
Reactivity
n/a
Source
CHO cells
Location
AANKRKNQNR NKSSSHQDSS RMSSVGDYNT SEQKQACKKH ELYVSFRDLG WQDWIIAPEG YAAFYCDGEC SFPLNAHMNA TNHAIVQTLV HLMFPDHVPK PCCAPTKLNA ISVLYFDDSS NVILKKYRNM VVRSCGCH
Species Reactivity
n/a
