Background
ANG-2bindstotheendothelialcellspecificreceptorTie2,but,incontrasttoANG-1doesnotinducetyrosinephosphorylation.Consequently,ANG-2modulatesANG-1activationofTie2and,dependingonthephysiologicalandbiochemicalenvironment,canacteitherasanagoNISTorantagonistofTie2inducedangiogenesis.ThesignalinginteractionsofANG-1,ANG-2andTie2,alongwithlesscharacterizedANG-3andANG-4,arerequiredforembryonicandadultangiogenesis.Physiologically,ANG-1andANG-2areassociatedwithsprouting,tubeformation,andstructuralintegrityofnewlyformedbloodvessels.MaturehumanANG-2isasecretedproteincontaining480aminoacidresidues.ANG-2iscomposedofanalphahelixrich¡°coiledcoil¡±N-terminaldomainandfibrinogenlikeC-terminaldomain.ANG-2existspredominantlyintheformofadisulfide-linkeddimer.RecombinanthumanANG-2isaC-terminalhistidinetaggedglycoproteinwhichmigrateswithanapparentmolecularmassof60.0-70.0kDabySDS-PAGEunderreducingconditions.SequencinganalysisshowsanN-terminalsequencestartingwithresidue68(D)oftheANG-2precursorprotein.
Specifications
AdditionalNames
ANGPT2TestedApplication
n/aDisclaimer
Thisproductisforresearchuseonly.Purity
Greaterthan95%bySDS-PAGEgelandHPLCanalyses.Storage
ThelyophilizedANG-2recombinantproteinisstableforatleast2yearsfromdateofreceiptat-20ËšC.ReconstitutedANG-2isstableforatleast3monthswhenstoredinworkingaliquotswithacarrierproteinat-20ËšC.Aswithanyprotein,exposingYeastANG-2recombinantproteintorepeatedfreeze/thawcyclesisnotrecommended.Whenworkingwithproteinscareshouldbetakentokeeprecombinantproteinatacoolandstabletemperature.Preparation
BIOLOGicalactivitywasdeterminedbyitsABIlitytostimulatetubulogenesisinHUVECcellsusingaconcentrationof0.2μg/mL.Endotoxinlevelislessthan0.1ngperμg(1EU/μg).Reactivity
n/aSource
CHOcellsLocation
DAPLEYDDSVQRLQVLENIMENNTQWLMKLENYIQDNMKKEMVEIQQNAVQNQTAVMIEIGTNLLNQTAEQTRKLTDVEAQVLNQTTRLELQLLEHSLSTNKLEKQILDQTSEINKLQDKNSFLEKKVLAMEDKHIIQLQSIKEEKDQLQVLVSKQNSIIEELEKKIVTATVNNSVLQKQQHDLMETVNNLLTMMSTSNSAKDPTVAKEEQISFRDCAEVFKSGHTTNGIYTLTFPNSTEEIKAYCDMEAGGGGWTIIQRREDGSVDFQRTWKEYKVGFGNPSGEYWLGNEFVSQLTNQQRYVLKIHLKDWEGNEAYSLYEHFYLSSEELNYRIHLKGLTGTAGKISSISQPGNDFSTKDGDNDKCICKCSQMLTGGWWFDACGPSNLNGMYYPQRQNTNKFNGIKWYYWKGSGYSLKATTMMIRPADFHHHHHHSpeciesReactivity
n/a