MouseCerebralCortex,frontaltotalprotein(proteinlysate)isisolatedfromfreshlyharvestedbrains.Tissuesarehomogenizedinproteinlysisbuffersupplementedwithacocktailof7mammalianproteaseinhibitorstominimizeproteolysis,preciselyquantified,andstoredat-80oC.
QualityControl: Qualityofproteinasindicatedbytheabsenceofsmear(nodegradation)andsharpnessandresolutionoftheproteinbandsisverifiedbydenaturedSDS-PAGEusingCoomassiebluestaining.Theintegrityofproteinistestedbyimmunoblottingusingspecificbeta-actinantibody.
Applications: TotalproteinisreadyforimmediateuseinWesternblotting,immunoprecipitation,SDS-PAGE,isoelectricfocusinggelsandSDS-capillaryelectrophoresis,enzymaticactivityanalysis,gelshiftingassay,protein-proteininteraction,andtissuespecificexpression.
Packing/shipping:Theextractedproteinisprovidedinvialsataconcentrationof5mg/mlandshippedondryice.ProteincanalsobeprovidedindenaturedSDSSamplebufferforonlyWesternblottinguse
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