MouseUniversalReference CDNA-RandomPrime isa fulllengthfirststrandcDNAsynthesizedfromZyagenuniversalreferencetotalRNAwhichis isolatedfromalltissuesofseveraladultmaleandfemaleC57BL/6miceusing modifiedguanidinethiocyanatetechniques.
ThecDNAisprimedwithRandomHexamerprimerandcanbeusedingenecloning,targetsequencingandasacontrolforgeneexpressionanalysisbyregularPCRandrealtimePCR. EachcDNAproductisenoughfor40PCRreactions.
Features:
- SynthesizedfromhighqualityintactuniversalreferencetotalRNA
- Consistentcontrolforstandarddatasetcomparisons
- Preparedfromallmousetissuesforbroadgenecoverage
- Madeat industrialscalesto minimizelot-to-lotvariation.
QualityControl: TheintegrityofeachRNAsampleusedforcDNAsynthesisisverifiedbydenaturedagarosegelelectrophoresis.ThepurityofRNAisassessedbyspectrophotometer(A260/A280:1.9-2.1).ResidualDNAcontaminationistestedbyPCR.
ThesynthesizedcDNAisalsousedastemplatePCRamplificationofß-actingene.PCRproductofß-actinwasvisualizedon1%agarosegel.
Applications: cDNAisidealasacontrolforgeneexpressionanalysisbyrealtimeandregularPCR,characterizationofalternativesplicingofmRNA,verificationofgeneticmutation,genecloningandtargetsequencing.
Packing/shipping:eachuniversalreferencecDNAsampleisroutinelyshippedondryicein1.5mlvialsandisenoughfor40reactions(40PCRamplifications).
MSDSandCertificateofAnalysisinPDFfiles: ContactZyagenTechnicalSupportat zinfo@zyagen.com