RatfulllengthtissueCDNApanelisyourchoiceofany9placentacDNAsamples(10reactionseach).
ThefulllengthtissuecDNApanelismadeofRNAextractedfromfreshlyharvestedplacentasusingclassicalguanidineisothiocyanate-phenol:chloroformextractionmethod. RNA istreatedwithRNase-freeDNase-1toremoveresidualDNA.ThecDNAisprimedwitholigodTprimer.
QualityControl: TheintegrityofeachRNAsampleasindicatedbyintactribosomalRNAisverifiedbydenaturedagarosegelelectrophoresis.ThepurityofRNAisassessedbyspectrophotometer(A260/A280:1.9-2.1).ResidualDNAcontaminationistestedbyPCR.
ThesynthesizedcDNAisalsotestedastemplateforPCRamplificationofß-actingene.PCRproductofß-actinwasvisualizedon1%agarosegel.
Applications: cDNAisidealforgeneexpressionanalysisbyPCR,characterizationofalternativesplicingofmRNA,verificationofgeneticmutation,genecloningandtargetsequencing.
Packing/shipping:eachcDNAsampleisroutinelyshippedondryicein1.5mlvials.
MSDSandCertificateofAnalysisinPDFfiles: ContactZyagenTechnicalSupportat zinfo@zyagen.com