MouseStriatumfulllengthCDNAismadeofRNAextractedfromfreshlyharvestedbrainusingclassicalguanidineisothiocyanate-phenol:chloroformextractionmethod. RNA istreatedwithRNase-freeDNase-1toremoveresidualDNA.ThecDNAisprimedwitholigodTprimer.
QualityControl:TheintegrityofeachRNAsampleasindicatedbyintactribosomalRNAisverifiedbydenaturedagarosegelelectrophoresis.ThepurityofRNAisassessedbyspectrophotometer(A260/A280:1.9-2.1).ResidualDNAcontaminationistestedbyPCR.
ThesynthesizedcDNAisalsousedastemplateforPCRamplificationofß-actingene.PCRproductofß-actinwasvisualizedon1%agarosegel.
Applications:cDNAisidealforgeneexpressionanalysisbyPCR,characterizationofalternativesplicingofmRNA,verificationofgeneticmutation,genecloningandtargetsequencing.
Packing/shipping:eachcDNAsampleisroutinelyshippedondryicein1.5mlvialsandisenoughfor30reactions(30PCRamplifications).
MSDSandCertificateofAnalysisinPDFfiles:ContactZyagenTechnicalSupportatzinfo@zyagen.com