MouseBrainstemTotalRNAisextractedfromfreshlyharvestedtissueusingclassicalguanidineisothiocyanate–phenol:chloroformextractionmethodwhichallowstherapidisolationoftotalRNAincludingmicroRNAs. RNA istreatedwithRNase-freeDNase-1toremoveresidualDNA,preciselyquantified,andstoredat-80oC.
QualityControl:TheintegrityofeachRNAsampleasindicatedbyintactribosomalRNAisverifiedbydenaturedagarosegelelectrophoresis.ThepurityofRNAisassessedbyspectrophotometer(A260/A280:1.9-2.1).ResidualDNAcontaminationistestedbyPCR.
Applications:RNAisidealforNorthernblotting,ribonucleaseprotectionassay,SInucleaseassay,RT-PCR/Q-PCR/RACEanalysis,CDNAsynthesis,RNAdifferentialdisplay,microRNAstudies,andpurificationofmRNAforlibraryconstruction.
Packing/shipping:TotalRNAsampleisroutinelyprovidedinRNase-freewaterataconcentrationof1mg/mlandshippedondryice.Samplecanalsobeprovidedin1mMsodiumcitrate,0.1mMEDTA,oranyotherRNAstoragebuffer.
MSDSandCertificateofAnalysisinPDFfiles:ContactZyagenTechnicalSupportatzinfo@zyagen.com